Three cases of double infection with elephant endotheliotropic herpesvirus (EEHV) types 1A and 4 Metal hair roller in captive Asian elephants are presented.The first calf was a 4-year-old female that showed initial signs of lethargy and depression.The second calf was a 6-year-old female that displayed signs of depression and diarrhea and died within 48 h of the start of supportive treatment.The third was TIMER a 2-year-old male that died suddenly while living with the herd.
Necropsies were performed in the first and second elephants, while only a tongue sample was collected from the third calf.EEHV infection was confirmed via quantitative PCR (qPCR) and gene sequencing, revealing double subtypes of EEHV1A and -4 infections.This study describes the hematological and pathological characteristics within the host following double EEHV infection.